Recombinant Human Crossover junction endonuclease MUS81 (MUS81)

Uniprot Id

Q96NY9

Target Species

Human

Target Name

MUS81

Target Full Name

Crossover junction endonuclease MUS81

Target Function

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Target Subcellular Location

Nucleus, nucleolus. Note=Recruited to foci of DNA damage in S-phase cells.

Target Protein Families

XPF family

Target Tissue Specificity

Widely expressed.

Target Synonyms

Crossover junction endonuclease MUS81; FLJ21012; FLJ44872; Mus 81; MUS81; MUS81 Endonuclease Homolog ; MUS81_HUMAN

Target Background

This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.