Recombinant Human Cyclin-dependent kinase 4 (CDK4)

The gene encoding the Human CDK4 protein (2-303aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human CDK4 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human CDK4 protein, revealing a purity level exceeding 90%.

ACP03665

Number

Cat.No	ACP03665	Target Name	CDK4
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	2-303aa	Mol Weight	37.6kDa
Protein Length	Full Length of Mature Protein	Purity	Greater than 90% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP03665

Target Name

CDK4

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

2-303aa

Mol Weight

37.6kDa

Protein Length

Full Length of Mature Protein

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P11802

Background Information

Uniprot Id

P11802
Target Species

Human
Target Name

CDK4
Target Full Name

Cyclin-dependent kinase 4
Target Function

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Target Involvement

Melanoma, cutaneous malignant 3 (CMM3)
Target Subcellular Location

Cytoplasm. Nucleus. Nucleus membrane.
Target Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Target Research Area

Cancer, Cell Cycle
Target Synonyms

Cdk 4; cdk4; CDK4 protein; CDK4_HUMAN; Cell division kinase 4; Cell division protein kinase 4; CMM 3; CMM3; Crk3; Cyclin dependent kinase 4; Cyclin-dependent kinase 4; Melanoma cutaneous malignant 3; MGC14458; p34 cdk4; PSK J3; PSK-J3
Target Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Recombinant Human Cyclin-dependent kinase 4 (CDK4)

Specifications

Immunogen Information

Inquire Recombinant Human Cyclin-dependent kinase 4 (CDK4) Now

Related Products