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Synthesizing the recombinant Human FLAD1 protein generally involves integrating the DNA fragment that encodes the Human FLAD1 protein (1-490aa) into a plasmid, introducing the recombinant plasmid into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. A N-terminal 6xHis tag is fused to the protein. The protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. The protein's purity surpasses 90%.
Synthesizing the recombinant Human FLAD1 protein generally involves integrating the DNA fragment that encodes the Human FLAD1 protein (1-490aa) into a plasmid, introducing the recombinant plasmid into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. A N-terminal 6xHis tag is fused to the protein. The protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. The protein’s purity surpasses 90%.
| Cat.No | ACP03236 | Target Name | FLAD1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-490aa | Mol Weight | 58.3 kDa |
| Protein Length | Full Length of Isoform 2 | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8NFF5 |
|---|
Uniprot Id
Q8NFF5
Target Species
Human
Target Name
FLAD1
Target Full Name
FAD synthase
Target Function
Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.
Target Involvement
Lipid storage myopathy due to flavin adenine dinucleotide synthetase deficiency (LSMFLAD)
Target Subcellular Location
[Isoform 1]: Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
Target Protein Families
MoaB/Mog family; PAPS reductase family, FAD1 subfamily
Target Research Area
Metabolism
Target Synonyms
FLAD1; PP591FAD synthase; EC 2.7.7.2; FAD pyrophosphorylase; FMN adenylyltransferase; Flavin adenine dinucleotide synthase) [Includes: Molybdenum cofactor biosynthesis protein-like region; FAD synthase region]
Target Background
This gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
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