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In the process of expressing the recombinant Human GAL3ST3 protein, e.coli cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (41-431aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human GAL3ST3 protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 85%, as assessed by SDS-PAGE.
In the process of expressing the recombinant Human GAL3ST3 protein, e.coli cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (41-431aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human GAL3ST3 protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 85%, as assessed by SDS-PAGE.
| Cat.No | ACP01744 | Target Name | GAL3ST3 |
|---|---|---|---|
| Target Synonyms | (Gal3ST-3)(Beta-galactose-3-O-sulfotransferase 3)(Gal3ST3)(Gal-beta-1, 3-GalNAc 3'-sulfotransferase 3) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 41-431aa |
| Mol Weight | 51.9 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q96A11 |
|---|
Uniprot Id
Q96A11
Target Species
Human
Target Name
GAL3ST3
Target Full Name
Galactose-3-O-sulfotransferase 3
Target Function
Transfers a sulfate to position 3 of non-reducing beta-galactosyl residues in N-glycans and core2-branched O-glycans. Has high activity towards Gal-beta-1,4-GlcNAc, Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc and lower activity towards Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc.
Target Subcellular Location
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.
Target Protein Families
Galactose-3-O-sulfotransferase family
Target Tissue Specificity
Highly expressed in thyroid, brain, kidney, heart and spinal cord.
Target Research Area
Others
Target Synonyms
GAL3ST3Galactose-3-O-sulfotransferase 3; Gal3ST-3; EC 2.8.2.-; Beta-galactose-3-O-sulfotransferase 3; Gal3ST3; Gal-beta-1; 3-GalNAc 3'-sulfotransferase 3
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