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To produce the recombinant human glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2) in the E. coli expression system, the gene fragment encoding the full-length mature GPD2 protein (43-727aa) is cloned into an appropriate expression vector. The vector also incorporates an N-terminal 6xHis-tag downstream of the gene fragment. The resulting recombinant plasmid is then introduced into E. coli host cells through a transformation process and the transformed cells are selected using suitable markers. The transformed E. coli cells are subsequently cultivated in a growth medium optimized for protein expression. To induce protein production, an inducer molecule like IPTG is added to the culture. Following an incubation period, the cells are harvested and lysed to release the intracellular contents containing the expressed recombinant GPD2 protein. Through SDS-PAGE analysis, the purified recombinant human GPD2 protein demonstrates a high purity level of up to 90%, evident by its migration as a distinct band with a molecular weight of approximately 90 kDa on the gel.
To produce the recombinant human glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2) in the E. coli expression system, the gene fragment encoding the full-length mature GPD2 protein (43-727aa) is cloned into an appropriate expression vector. The vector also incorporates an N-terminal 6xHis-tag downstream of the gene fragment. The resulting recombinant plasmid is then introduced into E. coli host cells through a transformation process and the transformed cells are selected using suitable markers. The transformed E. coli cells are subsequently cultivated in a growth medium optimized for protein expression. To induce protein production, an inducer molecule like IPTG is added to the culture. Following an incubation period, the cells are harvested and lysed to release the intracellular contents containing the expressed recombinant GPD2 protein. Through SDS-PAGE analysis, the purified recombinant human GPD2 protein demonstrates a high purity level of up to 90%, evident by its migration as a distinct band with a molecular weight of approximately 90 kDa on the gel.
| Cat.No | ACP01055 | Target Name | GPD2 |
|---|---|---|---|
| Target Synonyms | (GPD-M)(GPDH-M)(mitohondrial glycerophosphate dehydrogenase gene)(mGDH)(mtGPD) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 43-727aa |
| Mol Weight | 82.3 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P43304 |
|---|
Uniprot Id
P43304
Target Species
Human
Target Name
GPD2
Target Full Name
Glycerol-3-phosphate dehydrogenase, mitochondrial
Target Function
Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device.
Target Subcellular Location
Mitochondrion.
Target Protein Families
FAD-dependent glycerol-3-phosphate dehydrogenase family
Target Research Area
Others
Target Synonyms
GDH2; Glycerol 3 phosphate dehydrogenase 2 (mitochondrial); Glycerol 3 phosphate dehydrogenase 2; Glycerol 3 phosphate dehydrogenase mitochondrial; Glycerol-3-phosphate dehydrogenase; GPD 2; GPD M; GPD-M; gpd2; GPDH M; GPDH-M; GPDM; GPDM_HUMAN; mGPDH; mitochondrial; Mitochondrial glycerophosphate dehydrogenase; mtGPD; OTTHUMP00000162788; OTTHUMP00000204452; OTTHUMP00000204453; OTTHUMP00000204458
Target Background
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
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