Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The gene fragment corresponding to the 117-269aa of the human IL1B protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with N-terminal 6xHis-SUMO tag. The E.coli was transformed with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as N-terminal 6xHis-SUMO-tagged fusion. Its purity is greater than 90% assayed by SDS-PAGE. The IL1B protein ran to a band of about 33 kDa molecular weight on the gel.Interleukin 1beta is a proinflammatory cytokine encoded by the IL1B gene located on chromosome 2. Interleukin levels are normally low in the CNS but are elevated after acute injury and in chronic neurodegenerative diseases such as Alzheimer's disease (AD). IL1 gene variants should be considered disease modifiers in RA. Some researches found that he rare allele of the IL-1B is associated with the destructive pattern characteristic of chronic RA. The pro-inflammatory cytokine IL-1 is produced by the inflammatory synovium and appears to play a predominant role in the destructive pattern associated with RA. It is a known activator of collagenase and stromelysin and inhibits the synthesis of collagen and proteoglycan. In addition, p62-induced autophagic degradation of caspase-1 accounted for caspase-1/IL1B inactivation in CD44s deficient cells. Many findings implied that IL1B polymorphisms modulate cystic fibrosis lung disease.
The gene fragment corresponding to the 117-269aa of the human IL1B protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with N-terminal 6xHis-SUMO tag. The E.coli was transformed with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as N-terminal 6xHis-SUMO-tagged fusion. Its purity is greater than 90% assayed by SDS-PAGE. The IL1B protein ran to a band of about 33 kDa molecular weight on the gel.Interleukin 1beta is a proinflammatory cytokine encoded by the IL1B gene located on chromosome 2. Interleukin levels are normally low in the CNS but are elevated after acute injury and in chronic neurodegenerative diseases such as Alzheimer’s disease (AD). IL1 gene variants should be considered disease modifiers in RA. Some researches found that he rare allele of the IL-1B is associated with the destructive pattern characteristic of chronic RA. The pro-inflammatory cytokine IL-1 is produced by the inflammatory synovium and appears to play a predominant role in the destructive pattern associated with RA. It is a known activator of collagenase and stromelysin and inhibits the synthesis of collagen and proteoglycan. In addition, p62-induced autophagic degradation of caspase-1 accounted for caspase-1/IL1B inactivation in CD44s deficient cells. Many findings implied that IL1B polymorphisms modulate cystic fibrosis lung disease.
| Cat.No | ACP02421 | Target Name | IL1B |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 117-269aa | Mol Weight | 33.4kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P01584 |
|---|
Uniprot Id
P01584
Target Species
Human
Target Name
IL1B
Target Full Name
Interleukin-1 beta
Target Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Target Subcellular Location
Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
Target Protein Families
IL-1 family
Target Tissue Specificity
Expressed in activated monocytes/macrophages (at protein level).
Target Research Area
Immunology
Target Synonyms
Catabolin; H1; IFN beta inducing factor; IL 1; IL 1 beta; IL-1 beta; IL1; IL1 BETA; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; interleukin 1; beta; Interleukin-1 beta; OAF; Osteoclast activating factor; OTTHUMP00000162031; Preinterleukin 1 beta; Preinterleukin beta; Pro interleukin 1 beta
Target Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
