Recombinant Human Isthmin-1 (ISM1)

Recombinant Human ISM1?protein is a prokaryotic system E.coli expressed protein (Full Length of Mature Protein). To make this ISM1?recombinant protein, the ISM1 gene is synthesized and cloned into the E.coli system expression vector, and then it was transformed into protein-expressing host E Coli for expression, the other steps are testing for the identification of recombinant protein, large scale production and target protein Isolation and purification. The purity of the final ISM1 protein is 85%+?as determined by SDS-PAGE.ISM1 (also known as ISM or C20f82) is a protein encoding gene that provides an instruction in making a protein named Isthmin-1. isthmin-1 is a 52-kDa secreted protein with a thrombospondin type 1 repeat (TSR) domain, containing two consensus C-mannosylation sequences at Trp223 and Trp226 in human. This protein has a dual role in increasing adipose glucose uptake while suppressing hepatic lipid synthesis as an adipokine. It is also essential during organ development and active in parts of the immune response.