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An ORF cDNA corresponding to the peptide of Human Melanocyte protein PMEL was expressed with an N-terminal 6xHis-tag in the yeast cells. PMEL CSB-YP021324HU is a truncated molecule with amino acids of Lys25-Val467. Its purity is greater than 90% assessed by SDS-PAGE. A molecular mass band of about 52 kDa was presented on the gel under reducing conditions. It was also validated by the LC-MS/MS analysis. This recombinant PMEL protein may be used to produce specific anti-PMEL antibodies or in the studies of PMEL-associated metabolism. Premelanosome Protein (PMEL) is an amyloid protein that participates in the melanosome matrix formation through the interaction with melanin. Eumelanin deposition requires the expression of PMEL and proper formation of the melanosome matrix, both of which are not crucial for the development of pheomelanic melanosomes. As a consequence, PMEL mutations lead to eumelanin defects in cattle, chicken, and mouse. PMEL mutations are also associated with pigmentary glaucoma in humans.
An ORF cDNA corresponding to the peptide of Human Melanocyte protein PMEL was expressed with an N-terminal 6xHis-tag in the yeast cells. PMEL CSB-YP021324HU is a truncated molecule with amino acids of Lys25-Val467. Its purity is greater than 90% assessed by SDS-PAGE. A molecular mass band of about 52 kDa was presented on the gel under reducing conditions. It was also validated by the LC-MS/MS analysis. This recombinant PMEL protein may be used to produce specific anti-PMEL antibodies or in the studies of PMEL-associated metabolism.
Premelanosome Protein (PMEL) is an amyloid protein that participates in the melanosome matrix formation through the interaction with melanin. Eumelanin deposition requires the expression of PMEL and proper formation of the melanosome matrix, both of which are not crucial for the development of pheomelanic melanosomes. As a consequence, PMEL mutations lead to eumelanin defects in cattle, chicken, and mouse. PMEL mutations are also associated with pigmentary glaucoma in humans.
| Cat.No | ACP04794 | Target Name | PMEL |
|---|---|---|---|
| Target Synonyms | homolog of, mouse | Form | Liquid or Lyophilized powder |
| Expression System | Yeast | Expression Range | 25-467aa |
| Mol Weight | 49.0kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P40967 |
|---|
Uniprot Id
P40967
Target Species
Human
Target Name
PMEL
Target Full Name
Melanocyte protein PMEL
Target Function
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Target Subcellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus. Melanosome. Endosome, multivesicular body. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.; [M-alpha]: Secreted.
Target Protein Families
PMEL/NMB family
Target Tissue Specificity
Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and
Target Research Area
Metabolism
Target Synonyms
95 kDa melanocyte specific secreted glycoprotein; 95 kDa melanocyte-specific secreted glycoprotein; D12S53E; gp100; M-beta; ME20; ME20 M/ME20 S; ME20-M; ME20-S; ME20M; ME20M/ME20S; ME20S; Melanocyte lineage specific antigen GP100 ; Melanocyte protein mel 17 ; Melanocyte protein Pmel 17; Melanocyte protein Pmel 17 precursor ; Melanocytes lineage-specific antigen GP100; Melanoma associated ME20 antigen; Melanoma gp100; Melanoma-associated ME20 antigen; Melanosomal matrix protein 17; Melanosomal matrix protein17; P1; p100; p26; PMEL 17; PMEL; PMEL_HUMAN; PMEL17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; SI; SIL; SILV; Silver (mouse homolog) like; Silver homolog; Silver locus protein homolog; Silver, mouse, homolog of
Target Background
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
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