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Expressing the recombinant Human MADCAM1 protein generally involves constructing a plasmid encoding the Human MADCAM1 protein (19-317aa). This plasmid is introduced into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. The protein is fused with a N-terminal 6xHis-SUMO tag. The recombinant Human MADCAM1 protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. This protein exhibits a purity exceeding 90%.
Expressing the recombinant Human MADCAM1 protein generally involves constructing a plasmid encoding the Human MADCAM1 protein (19-317aa). This plasmid is introduced into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. The protein is fused with a N-terminal 6xHis-SUMO tag. The recombinant Human MADCAM1 protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. This protein exhibits a purity exceeding 90%.
| Cat.No | ACP03757 | Target Name | MADCAM1 |
|---|---|---|---|
| Target Synonyms | Addressin mucosal; hMAdCAM 1 ; hMAdCAM-1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 19-317aa |
| Mol Weight | 47.4kDa | Protein Length | Extracellular Domain |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q13477 |
|---|
Uniprot Id
Q13477
Target Species
Human
Target Name
MADCAM1
Target Full Name
Mucosal addressin cell adhesion molecule 1
Target Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Target Subcellular Location
Membrane; Single-pass type I membrane protein.
Target Tissue Specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ov
Target Research Area
Immunology
Target Synonyms
Addressin mucosal; hMAdCAM 1 ; hMAdCAM-1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1
Target Background
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
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