Recombinant Human Myeloid cell surface antigen CD33 (CD33), Truncated

The gene encoding the Human CD33 protein (18-259aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 10xHis-SUMO tag and C-terminal Myc tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human CD33 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human CD33 protein, revealing a purity level exceeding 85%.

ACP02503

Number

Cat.No	ACP02503	Target Name	CD33
Target Synonyms	CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	48-259aa
Mol Weight	46.7 kDa	Protein Length	Extracellular Domain
Purity	Greater than 85% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP02503

Target Name

CD33

Target Synonyms

CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

48-259aa

Mol Weight

46.7 kDa

Protein Length

Extracellular Domain

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P20138

Background Information

Uniprot Id

P20138
Target Species

Human
Target Name

CD33
Target Full Name

Myeloid cell surface antigen CD33
Target Function

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Target Subcellular Location

[Isoform CD33M]: Cell membrane; Single-pass type I membrane protein.; [Isoform CD33m]: Peroxisome.
Target Protein Families

Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Target Tissue Specificity

Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells.
Target Research Area

Immunology
Target Synonyms

CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33
Target Background

Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.

Recombinant Human Myeloid cell surface antigen CD33 (CD33), Truncated

Specifications

Immunogen Information

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