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The formation of the recombinant plasmid by incorporating the gene encoding the Human PRSS12 protein (631-874aa) into a plasmid vector initiates the generation of the recombinant Human PRSS12 protein. Transforming the recombinant plasmid into e.coli cells and then selecting the positive e.coli cells based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that stimulate the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant Human PRSS12 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human PRSS12 protein, revealing a purity level exceeding 90%.
The formation of the recombinant plasmid by incorporating the gene encoding the Human PRSS12 protein (631-874aa) into a plasmid vector initiates the generation of the recombinant Human PRSS12 protein. Transforming the recombinant plasmid into e.coli cells and then selecting the positive e.coli cells based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that stimulate the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant Human PRSS12 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human PRSS12 protein, revealing a purity level exceeding 90%.
| Cat.No | ACP02451 | Target Name | PRSS12 |
|---|---|---|---|
| Target Synonyms | BSSP 3; BSSP3; Leydin; MGC12722; Motopsin; MRT1; NETR_HUMAN; Neurotrypsin; protease; serine; 12 (neurotrypsin; motopsin); Prss12; Serine protease 12 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 631-874aa |
| Mol Weight | 43.4kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P56730 |
|---|
Uniprot Id
P56730
Target Species
Human
Target Name
PRSS12
Target Full Name
Neurotrypsin
Target Function
Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations.
Target Involvement
Mental retardation, autosomal recessive 1 (MRT1)
Target Subcellular Location
Secreted.
Target Protein Families
Peptidase S1 family
Target Tissue Specificity
Brain and Leydig cells of the testis.
Target Research Area
Cell Biology
Target Synonyms
BSSP 3; BSSP3; Leydin; MGC12722; Motopsin; MRT1; NETR_HUMAN; Neurotrypsin; protease; serine; 12 (neurotrypsin; motopsin); Prss12; Serine protease 12
Target Background
This gene encodes a member of the trypsin family of serine proteases and contains a signal peptide, a proline-rich region, a Kringle domain, four scavenger receptor cysteine-rich domains, and a trypsin-like serine protease domain. The protein, sometimes referred to as neurotrypsin or motopsin, is secreted from neuronal cells and localizes to the synaptic cleft. Studies in mice show that this protein cleaves a protein, agrin, that is important for the formation and maintenance of exitatory synapses. Defects in this gene cause a form of autosomal recessive cognitive impairment (MRT1).
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