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The process for producing recombinant Human ASAH2 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human ASAH2 protein (610-780aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human ASAH2 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
The process for producing recombinant Human ASAH2 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human ASAH2 protein (610-780aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human ASAH2 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
| Cat.No | ACP01797 | Target Name | ASAH2 |
|---|---|---|---|
| Target Synonyms | N-CDase;NCDase;Acylsphingosine deacylase 2;BCDase;LCDase;hCD;N-acylsphingosine amidohydrolase 2;Non-lysosomal ceramidase | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 610-780aa |
| Mol Weight | 25.2 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9NR71 |
|---|
Uniprot Id
Q9NR71
Target Species
Human
Target Name
ASAH2
Target Full Name
Neutral ceramidase
Target Function
Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells. This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract.
Target Subcellular Location
[Neutral ceramidase]: Cell membrane; Single-pass type II membrane protein. Membrane raft; Single-pass type II membrane protein. Membrane, caveola; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Mitochondrion. Secreted, extracellular exosome.; [Neutral ceramidase soluble form]: Secreted.
Target Protein Families
Neutral ceramidase family
Target Tissue Specificity
Primarily expressed in intestine. Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart. The ubiquitous expression observed for ASAH2 might be an experimental artifact due to the paralog ASAH2B.
Target Research Area
Signal Transduction
Target Synonyms
Acylsphingosine deacylase 2; AI585898; ASAH2; ASAH2_HUMAN; BCDase; hCD; HNAC1; LCDase; MGC129777 ; mitochondrial ceramidase; N acylsphingosine amidohydrolase (non lysosomal ceramidase) 2; N acylsphingosine amidohydrolase 2; N CDase; N-acylsphingosine amidohydrolase 2; N-CDase; NCDase; Neutral ceramidase soluble form; Non lysosomal ceramidase; Non-lysosomal ceramidase
Target Background
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).
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