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Synthesizing the recombinant Human PITHD1 protein generally involves integrating the DNA fragment that encodes the Human PITHD1 protein (1-211aa) into a plasmid, introducing the recombinant plasmid into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. The protein's purity surpasses 90%.
Synthesizing the recombinant Human PITHD1 protein generally involves integrating the DNA fragment that encodes the Human PITHD1 protein (1-211aa) into a plasmid, introducing the recombinant plasmid into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. The protein’s purity surpasses 90%.
| Cat.No | ACP05142 | Target Name | PITHD1 |
|---|---|---|---|
| Target Synonyms | PITHD1; C1orf128; AD039; HT014; PP603; PITH domain-containing protein 1 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-211aa |
| Mol Weight | 31.6 kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9GZP4 |
|---|
Uniprot Id
Q9GZP4
Target Species
Human
Target Name
PITHD1
Target Full Name
PITH domain-containing protein 1
Target Function
Promotes megakaryocyte differentiation by up-regulating RUNX1 expression. Regulates RUNX1 expression by activating the proximal promoter of the RUNX1 gene and by enhancing the translation activity of an internal ribosome entry site (IRES) element in the RUNX1 gene.
Target Subcellular Location
Cytoplasm.
Target Protein Families
PITHD1 family
Target Tissue Specificity
Down-regulated in primary acute myeloid leukemia (AML) patients.
Target Research Area
Others
Target Synonyms
PITHD1; C1orf128; AD039; HT014; PP603; PITH domain-containing protein 1
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