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The expression region of this recombinant Human ATP4A covers amino acids 350-783. This ATP4A protein is theoretically predicted to have a molecular weight of 54.6 kDa. This ATP4A protein is produced using e.coli expression system. The ATP4A coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant ATP4A protein in following stages of expression and purification.The human potassium-transporting ATPase alpha chain 1 (ATP4A) is a subunit of the gastric H+/K+-ATPase, an enzyme crucial for the secretion of gastric acid into the stomach. ATP4A is primarily expressed in the parietal cells of the gastric mucosa and plays a key role in maintaining the acidic environment of the stomach, which is essential for digestion. ATP4A is involved in the active transport of protons (H+) into the stomach lumen, where they combine with chloride ions to form hydrochloric acid. This process is critical for the proper breakdown of ingested food. Dysfunction in ATP4A can lead to gastric acid-related disorders, such as peptic ulcers or gastroesophageal reflux disease (GERD). Understanding its function is vital for developing therapeutic strategies targeting acid-related gastrointestinal conditions.
The expression region of this recombinant Human ATP4A covers amino acids 350-783. This ATP4A protein is theoretically predicted to have a molecular weight of 54.6 kDa. This ATP4A protein is produced using e.coli expression system. The ATP4A coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant ATP4A protein in following stages of expression and purification.The human potassium-transporting ATPase alpha chain 1 (ATP4A) is a subunit of the gastric H+/K+-ATPase, an enzyme crucial for the secretion of gastric acid into the stomach. ATP4A is primarily expressed in the parietal cells of the gastric mucosa and plays a key role in maintaining the acidic environment of the stomach, which is essential for digestion. ATP4A is involved in the active transport of protons (H+) into the stomach lumen, where they combine with chloride ions to form hydrochloric acid. This process is critical for the proper breakdown of ingested food. Dysfunction in ATP4A can lead to gastric acid-related disorders, such as peptic ulcers or gastroesophageal reflux disease (GERD). Understanding its function is vital for developing therapeutic strategies targeting acid-related gastrointestinal conditions.
| Cat.No | ACP01194 | Target Name | ATP4A |
|---|---|---|---|
| Target Synonyms | (Gastric H(+)(+)(Proton pump) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 350-783aa |
| Mol Weight | 54.6 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P20648 |
|---|
Uniprot Id
P20648
Target Species
Human
Target Name
ATP4A
Target Full Name
Potassium-transporting ATPase alpha chain 1
Target Function
The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state
Target Subcellular Location
Apical cell membrane; Multi-pass membrane protein.
Target Protein Families
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Target Tissue Specificity
Expressed in gastric parietal cells (at protein level).
Target Research Area
Others
Target Synonyms
Atp4a; ATP4A_HUMAN; ATP6A; ATPase H+/K+ exchanging alpha polypeptide; ATPase H+/K+ transporting alpha polypeptide; Gastric H K ATPase alpha subunit; Gastric H K ATPase catalytic subunit; Gastric H(+)/K(+) ATPase subunit alpha; Gastric H+/K+ ATPase alpha subunit; Gastric hydrogen potassium ATPase; H(+) K(+) ATPase alpha subunit ; Potassium-transporting ATPase alpha chain 1; Proton pump
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