Recombinant Human Serine/arginine-rich splicing factor 3 (SRSF3), Truncated

The production of recombinant Human SRSF3 protein in e.coli cells is a multi-step process, including constructing the expression vector coding for the Human SRSF3 protein (1-86aa), transforming the recombinant vector into e.coli cells, selecting the cells that containing the vector and cultivating them, performing cell lysis, collecting the protein, and characterizing the protein. The resulting recombinant Human SRSF3 protein is purified from the cell lysate through affinity purification, and its purity, determined by SDS-PAGE, is greater than 90%.

ACP01945

Number

Cat.No	ACP01945	Target Name	SRSF3
Target Synonyms	arginine/serine-rich 3, Pre-mRNA-splicing factor SRP20;Splicing factor	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	1-86aa
Mol Weight	17.9 kDa	Protein Length	Partial
Purity	Greater than 90% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01945

Target Name

SRSF3

Target Synonyms

arginine/serine-rich 3, Pre-mRNA-splicing factor SRP20;Splicing factor

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-86aa

Mol Weight

17.9 kDa

Protein Length

Partial

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P84103

Background Information

Uniprot Id

P84103
Target Species

Human
Target Name

SRSF3
Target Full Name

Serine/arginine-rich splicing factor 3
Target Function

Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.
Target Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.
Target Protein Families

Splicing factor SR family
Target Research Area

Epigenetics and Nuclear Signaling
Target Synonyms

arginine/serine-rich 3; Pre mRNA splicing factor SRP20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor; Splicing factor arginine/serine rich 20 kD ; Splicing factor arginine/serine rich 3 ; SRp20; SRSF3; SRSF3_HUMAN; X16
Target Background

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene.

Recombinant Human Serine/arginine-rich splicing factor 3 (SRSF3), Truncated

Specifications

Immunogen Information

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