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The generation of the recombinant Human SRR protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human SRR protein (1-340aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant SRR protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human SRR protein, revealing a purity level exceeding 90%.
The generation of the recombinant Human SRR protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human SRR protein (1-340aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant SRR protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human SRR protein, revealing a purity level exceeding 90%.
| Cat.No | ACP03709 | Target Name | SRR |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-340aa | Mol Weight | 52.6kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9GZT4 |
|---|
Uniprot Id
Q9GZT4
Target Species
Human
Target Name
SRR
Target Full Name
Serine racemase
Target Function
Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
Target Protein Families
Serine/threonine dehydratase family
Target Tissue Specificity
Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
Target Research Area
Neuroscience
Target Synonyms
D serine ammonia lyase; D serine dehydratase; D-serine ammonia-lyase; D-serine dehydratase; ILV1; ISO1; L serine ammonia lyase; L serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; Serine racemase; srr; SRR_HUMAN
Target Background
Enables several functions, including L-serine ammonia-lyase activity; PDZ domain binding activity; and anion binding activity. Involved in pyruvate biosynthetic process; response to lipopolysaccharide; and serine family amino acid metabolic process. Located in cytoplasm and neuronal cell body.
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