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In the production of the recombinant Human SF3B3 protein in e.coli cells, a sequential pipeline involves constructing the expression vector coding for the Human SF3B3 protein (819-1217aa(T899I)), transforming the recombinant vector into e.coli cells, selecting the positive cells and cultivating them, lysing cells, and characterizing the protein. The resulting recombinant Human SF3B3 protein is purified from the cell lysate using affinity purification. Its purity exceeds 85%, as determined by SDS-PAGE.
In the production of the recombinant Human SF3B3 protein in e.coli cells, a sequential pipeline involves constructing the expression vector coding for the Human SF3B3 protein (819-1217aa(T899I)), transforming the recombinant vector into e.coli cells, selecting the positive cells and cultivating them, lysing cells, and characterizing the protein. The resulting recombinant Human SF3B3 protein is purified from the cell lysate using affinity purification. Its purity exceeds 85%, as determined by SDS-PAGE.
| Cat.No | ACP02091 | Target Name | SF3B3 |
|---|---|---|---|
| Target Synonyms | Pre-mRNA-splicing factor SF3b 130 kDa subunit (SF3b130) (STAF130) (Spliceosome-associated protein 130) (SAP 130) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 819-1217aa(T899I) |
| Mol Weight | 52.1 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q15393 |
|---|
Uniprot Id
Q15393
Target Species
Human
Target Name
SF3B3
Target Full Name
Splicing factor 3B subunit 3
Target Function
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Target Subcellular Location
Nucleus.
Target Protein Families
RSE1 family
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
KIAA0017; Pre mRNA splicing factor SF3b 130 kDa subunit; Pre-mRNA-splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; SAP130 ; SF3b130; SF3B3; SF3B3_HUMAN; Spliceosome associated protein 130 ; Spliceosome-associated protein 130; Splicing factor 3b subunit 3 130kD; Splicing factor 3B subunit 3; STAF130
Target Background
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
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