Recombinant Human Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial (OXCT1)

CUSABIO team inserts the gene coding for the Human OXCT1 protein (40-520aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human OXCT1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human OXCT1 protein, demonstrating a purity greater than 90%.

ACP04220

Number

Cat.No	ACP04220	Target Name	OXCT1
Target Synonyms	mitochondrial	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	40-520aa
Mol Weight	56.1kDa	Protein Length	Full Length of Mature Protein
Purity	Greater than 90% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP04220

Target Name

OXCT1

Target Synonyms

mitochondrial

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

40-520aa

Mol Weight

56.1kDa

Protein Length

Full Length of Mature Protein

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P55809

Background Information

Uniprot Id

P55809
Target Species

Human
Target Name

OXCT1
Target Full Name

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Target Function

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Target Involvement

Succinyl-CoA:3-oxoacid CoA transferase deficiency (SCOTD)
Target Subcellular Location

Mitochondrion matrix.
Target Protein Families

3-oxoacid CoA-transferase family
Target Tissue Specificity

Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
Target Research Area

Cancer
Target Synonyms

3 oxoacid CoA transferase 1; 3-oxoacid CoA-transferase 1; EC 2.8.3.5; OTTHUMP00000120012; OTTHUMP00000221550; OXCT; Oxct1; SCOT; Scot S; Scot-S; SCOT1_HUMAN; Somatic type succinyl CoA:3 oxoacid CoA transferase; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; Succinyl CoA:3 ketoacid CoA transferase; Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial; Succinyl CoA:3 oxoacid CoA transferase; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Target Background

This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.

Recombinant Human Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial (OXCT1)

Specifications

Immunogen Information

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