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The production of recombinant Human TAPBP protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (21-414aa) of foreign DNA is merged with an expression vector and subsequently introduced into e.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The resulting recombinant Human TAPBP protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.
The production of recombinant Human TAPBP protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (21-414aa) of foreign DNA is merged with an expression vector and subsequently introduced into e.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The resulting recombinant Human TAPBP protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.
| Cat.No | ACP01202 | Target Name | TAPBP |
|---|---|---|---|
| Target Synonyms | (TPN)(TPSN)(NGS-17)(TAP-associated protein)(TAP-binding protein) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 21-414aa |
| Mol Weight | 49.4 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O15533 |
|---|
Uniprot Id
O15533
Target Species
Human
Target Name
TAPBP
Target Full Name
Tapasin
Target Function
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Target Involvement
Bare lymphocyte syndrome 1 (BLS1)
Target Subcellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Target Tissue Specificity
Neutrophils, mostly in fully differentiated cells.
Target Research Area
Immunology
Target Synonyms
TAPBP; NGS17; TAPA; Tapasin; TPN; TPSN; NGS-17; TAP-associated protein; TAP-binding protein
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