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The recombinant Human TSHB protein is produced by constructing the recombinant plasmid encoding the Human TSHB protein (21-132aa), transforming recombinant plasmid into e.coli cells, screening the positive e.coli cells and culturing them, and inducing the protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. Following expression, the recombinant TSHB protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human TSHB protein. Its purity is greater than 90%.
The recombinant Human TSHB protein is produced by constructing the recombinant plasmid encoding the Human TSHB protein (21-132aa), transforming recombinant plasmid into e.coli cells, screening the positive e.coli cells and culturing them, and inducing the protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. Following expression, the recombinant TSHB protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human TSHB protein. Its purity is greater than 90%.
| Cat.No | ACP03966 | Target Name | TSHB |
|---|---|---|---|
| Target Synonyms | TSHBThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain; Thyrotropin alfa | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 21-132aa |
| Mol Weight | 28.9kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P01222 |
|---|
Uniprot Id
P01222
Target Species
Human
Target Name
TSHB
Target Full Name
Thyrotropin subunit beta
Target Function
Indispensable for the control of thyroid structure and metabolism.
Target Subcellular Location
Secreted.
Target Protein Families
Glycoprotein hormones subunit beta family
Target Research Area
Signal Transduction
Target Synonyms
TSHBThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain; Thyrotropin alfa
Target Background
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants.
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