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Recombination of a plasmid encoding the Human YARS1 protein (2-528aa) is the first step during the production the recombinant Human YARS1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is used to isolate and purify the recombinant Human YARS1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human YARS1 protein. Its purity exceeds 90%.
Recombination of a plasmid encoding the Human YARS1 protein (2-528aa) is the first step during the production the recombinant Human YARS1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is used to isolate and purify the recombinant Human YARS1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human YARS1 protein. Its purity exceeds 90%.
| Cat.No | ACP03075 | Target Name | YARS1 |
|---|---|---|---|
| Target Synonyms | CMTDIC; SYYC_HUMAN; Tyrosine tRNA ligase, cytoplasmic; Tyrosine tRNA ligase 1, cytoplasmic; Tyrosyl tRNA synthetase; Tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase, cytoplasmic; TyrRS; yars; YRS; YTS | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-528aa |
| Mol Weight | 86.0kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P54577 |
|---|
Uniprot Id
P54577
Target Species
Human
Target Name
YARS
Target Full Name
Tyrosine--tRNA ligase, cytoplasmic
Target Function
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Target Involvement
Charcot-Marie-Tooth disease, dominant, intermediate type, C (CMTDIC)
Target Subcellular Location
Cytoplasm.
Target Protein Families
Class-I aminoacyl-tRNA synthetase family
Target Research Area
Metabolism
Target Synonyms
CMTDIC; SYYC_HUMAN; Tyrosine tRNA ligase, cytoplasmic; Tyrosine tRNA ligase 1, cytoplasmic; Tyrosyl tRNA synthetase; Tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase, cytoplasmic; TyrRS; yars; YRS; YTS
Target Background
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
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