Recombinant Human Zona pellucida sperm-binding protein 3 (ZP3), Truncated

The recombinant Human ZP3 was expressed with the amino acid range of 23-387. This ZP3 protein is expected to have a theoretical molecular weight of 56.6 kDa. Expression of this ZP3 protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of ZP3, which enables a simple process of detecting and purifying the ZP3 recombinant protein in the following steps.The human zona pellucida sperm-binding protein 3 (ZP3) is a glycoprotein that plays a crucial role in the process of fertilization. ZP3 is a component of the zona pellucida, an extracellular matrix surrounding the oocyte. During fertilization, ZP3 serves as a sperm receptor, facilitating the binding and recognition of spermatozoa to the zona pellucida. This interaction triggers the acrosome reaction in sperm, enabling them to penetrate the zona pellucida and reach the oocyte for fertilization. ZP3 is essential for species-specific fertilization, as it ensures that only sperm of the same species can bind to and fertilize the egg. Additionally, ZP3 has been studied for its potential applications in contraceptive vaccine development, as antibodies against ZP3 can block sperm-egg interaction, providing a contraceptive effect.