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The antibody against ALKBH8 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against ALKBH8 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of various lysates, using ALKBH8 Rabbit pAb at 1:14000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-11777A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ALKBH8 |
| Target Synonyms | ABH8; TRM9; MRT71; TRMT9; TRMT9A; ALKBH8 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Rat brain, A549 | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q96BT7 | Immunogen Sequence |
Uniprot Id
Q96BT7
Target Species
Human
Target Name
ALKBH8
Target Full Name
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8
Target Function
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
Target Subcellular Location
Cytoplasm. Nucleus.
Target Protein Families
AlkB family
Target Tissue Specificity
Widely expressed, with highest expression in spleen, followed by pancreas and lung.
Target Synonyms
4930562C03Rik; 8030431D03Rik; 9430088N01Rik; ABH8; alkB alkylation repair homolog 8 (E. coli); alkB alkylation repair homolog 8; AlkB homologue 8; ALKB8_HUMAN; ALKBH8; Alkylated DNA repair protein alkB homolog 8; FLJ38204; MGC10235; OTTHUMP00000211286; OTTHUMP00000211287; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; RGD1304687; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8
Target Background
Enables tRNA (uracil) methyltransferase activity; tRNA binding activity; and zinc ion binding activity. Involved in cellular response to DNA damage stimulus; tRNA methylation; and tRNA wobble uridine modification. Located in cytosol and nuclear body. Implicated in autosomal recessive non-syndromic intellectual disability.
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