Recombinant Human Protein sprouty homolog 2 (SPRY2)

In the process of producing the recombinant Human SPRY2 protein, e.coli cells are transfected with a DNA expression vector containing the gene for the desired protein (1-315aa). Subsequent culturing induces the expression of the intended protein. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. The recombinant Human SPRY2 protein is then collected and purified from the cell lysate through affinity purification, displaying a purity exceeding 85%, as verified by SDS-PAGE.

ACP01312

Number

Cat.No	ACP01312	Target Name	SPRY2
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	1-315aa	Mol Weight	42.1 kDa
Protein Length	Full length	Purity	Greater than 85% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01312

Target Name

SPRY2

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-315aa

Mol Weight

42.1 kDa

Protein Length

Full length

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	O43597

Background Information

Uniprot Id

O43597
Target Species

Human
Target Name

SPRY2
Target Full Name

Protein sprouty homolog 2
Target Function

Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2. Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis. Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells. Inhibits CBL/C-CBL-mediated EGFR ubiquitination.
Target Involvement

IgA nephropathy 3 (IGAN3)
Target Subcellular Location

Cytoplasm, cytoskeleton. Cell projection, ruffle membrane.
Target Protein Families

Sprouty family
Target Research Area

Cardiovascular
Target Synonyms

hSPRY 2; hSPRY2; MGC23039; Protein sprouty homolog 2; Sprouty 2; Sprouty homolog 2 (Drosophila); Sprouty homolog 2; Sprouty2; Spry 2; Spry-2; SPRY2; SPY2_HUMAN
Target Background

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Recombinant Human Protein sprouty homolog 2 (SPRY2)

Specifications

Immunogen Information

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