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The process for recombinant Human TPP2 protein synthesis in e.coli cells includes recombining the DNA fragment that encodes the Human TPP2 protein (44-264aa) into expression vector, transforming the recombinant vector into e.coli cells, screening positive cells, cultivating and inducing cells, conducting cell lysis, and performing expression analysis. The resulting recombinant Human TPP2 protein is purified from the cell lysate using affinity purification, with its purity exceeding 90% as determined by SDS-PAGE.
The process for recombinant Human TPP2 protein synthesis in e.coli cells includes recombining the DNA fragment that encodes the Human TPP2 protein (44-264aa) into expression vector, transforming the recombinant vector into e.coli cells, screening positive cells, cultivating and inducing cells, conducting cell lysis, and performing expression analysis. The resulting recombinant Human TPP2 protein is purified from the cell lysate using affinity purification, with its purity exceeding 90% as determined by SDS-PAGE.
| Cat.No | ACP04958 | Target Name | TPP2 |
|---|---|---|---|
| Target Synonyms | TPP2; Tripeptidyl-peptidase 2; TPP-2; EC 3.4.14.10; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 44-264aa |
| Mol Weight | 31.8 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P29144 |
|---|
Uniprot Id
P29144
Target Species
Human
Target Name
TPP2
Target Full Name
Tripeptidyl-peptidase 2
Target Function
Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Stimulates adipogenesis.
Target Subcellular Location
Cytoplasm. Nucleus. Note=Translocates to the nucleus in responce to gamma-irradiation.
Target Protein Families
Peptidase S8 family
Target Research Area
Others
Target Synonyms
TPP2; Tripeptidyl-peptidase 2; TPP-2; EC 3.4.14.10; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II
Target Background
This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
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