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The antibody against ipaD was raised in rabbit using the Recombinant Shigella flexneri Invasin IpaD (IpaD) (1-332aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein G Purified. This antibody has been validated on ELISA, WB.
The antibody against ipaD was raised in rabbit using the Recombinant Shigella flexneri Invasin IpaD (IpaD) (1-332aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein G Purified. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in Recombinant protein | All lanes: IPAD antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 52.7 kDa | Observed band size: 56 kDa
| Cat.No | ADC-55274A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ipaD |
| Target Synonyms | Invasin IpaD (36 kDa membrane antigen), ipaD | Form | Liquid |
| Species Reactivity | Shigella flexneri | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Shigella flexneri Invasin IpaD (IpaD) (1-332aa) | Target Species | Shigella flexneri |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P18013 |
Uniprot Id
P18013
Target Species
Shigella flexneri
Target Name
IPAD
Target Function
Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via TTSS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.
Target Subcellular Location
Secreted. Note=Secreted via the type III secretion system (TTSS). Localizes to the tip of the external secretion needle that is part of the TTSS apparatus.
Target Protein Families
Invasin protein D family
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