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The antibody against AGO4 was raised in rabbit using the Recombinant Arabidopsis thaliana AGO4 protein (101-360aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
The antibody against AGO4 was raised in rabbit using the Recombinant Arabidopsis thaliana AGO4 protein (101-360aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
$299.00
Western Blot | Positive WB detected in: recombinant protein | All lanes: AGO4 Antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 33 kDa | Observed band size: 33 kDa
| Cat.No | ADC-55430A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | AGO4 |
| Target Synonyms | Protein argonaute 4 (Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11) AGO4 OCP11 At2g27040 T20P8.9 | Form | Liquid |
| Species Reactivity | Arabidopsis thaliana | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | >95%, Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Immunogen Description | Recombinant Arabidopsis thaliana AGO4 protein (101-360aa) | Target Species | Arabidopsis thaliana (Mouse-ear cress) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q9ZVD5 |
Uniprot Id
Q9ZVD5
Target Species
Arabidopsis thaliana
Target Name
AGO4
Target Function
Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci. Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses.
Target Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus, Cajal body.
Target Protein Families
Argonaute family, Ago subfamily
Target Tissue Specificity
Expressed in embryos, mature leaves, vascular tissue of the sepals, stamens and stigma, at the tip of the style and siliques.
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