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The antibody against recB was raised in rabbit using the Recombinant Escherichia coli (strain K12) recB protein (210-420aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
The antibody against recB was raised in rabbit using the Recombinant Escherichia coli (strain K12) recB protein (210-420aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in: recombinant protein | All lanes: recB Antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 29 kDa | Observed band size: 29 kDa
| Cat.No | ADC-55336A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | recB |
| Form | Liquid | Species Reactivity | Escherichia coli (strain K12) |
| Isotype | IgG | Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 |
| Purification Method | >95%, Protein G purified | Conjugate | Non-conjugated |
| Application | ELISA, WB |
| Immunogen Description | Recombinant Escherichia coli (strain K12) recB protein (210-420aa) | Target Species | Escherichia coli (strain K12) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P08394 |
Uniprot Id
P08394
Target Species
E.coli
Target Name
RECB
Target Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA. The RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions.
Target Protein Families
Helicase family, UvrD subfamily
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