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The antibody against AGMAT was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human AGMAT (NP_079034.3) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.
The antibody against AGMAT was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human AGMAT (NP_079034.3) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.
Western blot analysis of various lysates, using AGMAT Rabbit mAb at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Negative control (NC):HAP1
Exposure time: 10s.
| Cat.No | ADA-15608A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | AGMAT |
| Target Synonyms | AGMAT | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Hep G2 | Application | ELISA, WB, IF/ICC, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human AGMAT (NP_079034.3). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q9BSE5 | Immunogen Sequence |
Uniprot Id
Q9BSE5
Target Species
Human
Target Name
AGMAT
Target Full Name
Guanidino acid hydrolase, mitochondrial
Target Subcellular Location
Mitochondrion.
Target Protein Families
Arginase family, Agmatinase subfamily
Target Tissue Specificity
Highly expressed in liver and kidney. Also found in skeletal muscle, fetal liver, brain, testis, skin and the gastrointestinal tract. Within brain, expression is higher in the cerebral cortex with lower levels in the medulla and spinal cord.
Target Synonyms
AGMAT; Agmatinase; mitochondrial; EC 3.5.3.11; Agmatine ureohydrolase; AUH
Target Background
Predicted to enable agmatinase activity. Predicted to be involved in putrescine biosynthetic process from arginine, using agmatinase. Predicted to be located in mitochondrion.
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