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The antibody against CD123 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 20-305 of human CD123 (NP_002174.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against CD123 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 20-305 of human CD123 (NP_002174.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of Mouse spleen, using CD123 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
| Cat.No | ADA-07967A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CD123 |
| Target Synonyms | IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra; IL-3R-alpha | Form | Liquid |
| Species Reactivity | Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse spleen | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 20-305 of human CD123 (NP_002174.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P26951 | Immunogen Sequence |
Uniprot Id
P26951
Target Species
Human
Target Name
IL3RA
Target Full Name
Interleukin-3 receptor subunit alpha
Target Function
This is a receptor for interleukin-3.
Target Subcellular Location
Membrane; Single-pass type I membrane protein.
Target Protein Families
Type I cytokine receptor family, Type 5 subfamily
Target Synonyms
IL3RA; IL3R; Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; CD antigen CD123
Target Background
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
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