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The antibody against V5-Tag was raised in mouse using the GKPIPNPLLGLDST synthetic peptide conjugate to KLH as the immunogen. This antibody exists as a non-conjugated isotype IgG2a, purified by protein A with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, IP.
The antibody against V5-Tag was raised in mouse using the GKPIPNPLLGLDST synthetic peptide conjugate to KLH as the immunogen. This antibody exists as a non-conjugated isotype IgG2a, purified by protein A with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, IP.
$200.00
Western Blot Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng All lanes: V5 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa Observed band size: 32, 21, 55, 28, 58, 33 KDa Exposure time: 5min
Western Blot Positive WB detected in: 50ng V5-tagged fusion protein V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time: 5min2
Western Blot Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000 Lane 1: Recombinant V5-tagged fusion protein at 25ng Lane 2: Recombinant V5-tagged fusion protein at 12.5ng Lane 3: Recombinant V5-tagged fusion protein at 6.25ng Lane 4: Recombinant V5-tagged fusion protein at 3.125ng Lane 5: Recombinant V5-tagged fusion protein at 1.56ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 32 kd Observed band size: 32 kd Exposure time: 5min3
| Cat.No | ADC-57237A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Mouse | Target Name | V5-Tag |
| Form | Liquid | Species Reactivity | ALL |
| Isotype | IgG2a | Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 |
| Purification Method | >95%, Protein A purified | Conjugate | Non-conjugated |
| Application | ELISA, IF, WB, IP | Storage | Upon receipt |
| Immunogen Description | GKPIPNPLLGLDST synthetic peptide conjugate to KLH | Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
|---|
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