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Insertion of the gene encoding the Human ACAA1 protein (27-331aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. Following expression, the recombinant Human ACAA1 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ACAA1 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
Insertion of the gene encoding the Human ACAA1 protein (27-331aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. Following expression, the recombinant Human ACAA1 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ACAA1 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
| Cat.No | ACP04319 | Target Name | ACAA1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 27-331aa | Mol Weight | 47.9kDa |
| Protein Length | Full Length of Mature Protein of Isoform 2 | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P09110 |
|---|
Uniprot Id
P09110
Target Species
Human
Target Name
ACAA1
Target Full Name
3-ketoacyl-CoA thiolase, peroxisomal
Target Function
Responsible for the thiolytic cleavage of straight chain 3-oxoacyl-CoAs. Catalyzes the cleavage of short, medium and long straight chain 3-oxoacyl-CoAs, medium chain 3-oxoacyl-CoAs being the best substrates.
Target Subcellular Location
Peroxisome.
Target Protein Families
Thiolase family
Target Research Area
Metabolism
Target Synonyms
3 ketoacyl CoA thiolase peroxisomal; 3-ketoacyl-CoA thiolase; ACAA; ACAA1; Acetyl CoA acyltransferase 1; Acetyl Coenzyme A acyltransferase 1; Acetyl-CoA acyltransferase; Acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3 oxoacyl Coenzyme A; Beta ketothiolase; Beta-ketothiolase; Peroxisomal 3 oxoacyl CoA thiolase; Peroxisomal 3 oxoacyl Coenzyme A thiolase; Peroxisomal 3-oxoacyl-CoA thiolase; peroxisomal; PTHIO; THIK_HUMAN; THIO
Target Background
This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants.
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