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Expressing the recombinant Human ADAMTS7 protein in e.coli cells involves inserting a DNA fragment encoding the Human ADAMTS7 protein (242-593aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the ADAMTS7 protein. The protein carries a N-terminal 6xHis-SUMO tag. The cells are lysed to collect the recombinant Human ADAMTS7 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human ADAMTS7 protein exceeds 90%.
Expressing the recombinant Human ADAMTS7 protein in e.coli cells involves inserting a DNA fragment encoding the Human ADAMTS7 protein (242-593aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the ADAMTS7 protein. The protein carries a N-terminal 6xHis-SUMO tag. The cells are lysed to collect the recombinant Human ADAMTS7 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human ADAMTS7 protein exceeds 90%.
| Cat.No | ACP03147 | Target Name | ADAMTS7 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 242-593aa | Mol Weight | 52.1 kDa |
| Protein Length | Partial | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9UKP4 |
|---|
Uniprot Id
Q9UKP4
Target Species
Human
Target Name
ADAMTS7
Target Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 7
Target Function
Metalloprotease that may play a role in the degradation of COMP.
Target Subcellular Location
Secreted, extracellular space, extracellular matrix.
Target Tissue Specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
Target Research Area
Cell Biology
Target Synonyms
A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7; A disintegrin like and metalloprotease with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein; ADAM TS 7; ADAM TS7; ADAM-TS 7; ADAM-TS7; ADAMTS 7; ADAMTS-7; Adamts7; ATS7_HUMAN; COMPase; DKFZp434H204
Target Background
The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and may regulate vascular smooth muscle cell (VSMC) migration. Mutations in this gene may be associated with susceptibility to coronary artery disease.
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