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Amino acids 2-181 constitute the expression domain of recombinant Human ARF1. The calculated molecular weight for this ARF1 protein is 55.7 kDa. This ARF1 recombinant protein is manufactured in e.coli. The N-terminal 10xHis-GST tag and C-terminal Myc tag was fused into the coding gene segment of ARF1, making it easier to detect and purify the ARF1 recombinant protein in the later stages of expression and purification.ADP-ribosylation factor 1 (ARF1) is a critical protein involved in intracellular membrane transport and signal transduction. Some studies indicates that ARF1 plays a crucial role in the highly dynamic endoplasmic reticulum-Golgi apparatus-cell membrane system. Actually, ARF1 stands out for its role in controlling the secretion pathway. As a GTPase, ARF1 controls the formation and fusion of intracellular membrane vesicles by transitioning between active and inactive states on the membrane. This is vital for fundamental cellular processes such as intracellular protein transport, secretion, and endocytosis. In addition, ARF1 is implicated in regulating the dynamic balance of the cell cytoskeleton and cell polarity. It influences the invasion and metastasis of tumor cells and plays a role in synapse formation in neurons.
Amino acids 2-181 constitute the expression domain of recombinant Human ARF1. The calculated molecular weight for this ARF1 protein is 55.7 kDa. This ARF1 recombinant protein is manufactured in e.coli. The N-terminal 10xHis-GST tag and C-terminal Myc tag was fused into the coding gene segment of ARF1, making it easier to detect and purify the ARF1 recombinant protein in the later stages of expression and purification.ADP-ribosylation factor 1 (ARF1) is a critical protein involved in intracellular membrane transport and signal transduction. Some studies indicates that ARF1 plays a crucial role in the highly dynamic endoplasmic reticulum-Golgi apparatus-cell membrane system. Actually, ARF1 stands out for its role in controlling the secretion pathway. As a GTPase, ARF1 controls the formation and fusion of intracellular membrane vesicles by transitioning between active and inactive states on the membrane. This is vital for fundamental cellular processes such as intracellular protein transport, secretion, and endocytosis. In addition, ARF1 is implicated in regulating the dynamic balance of the cell cytoskeleton and cell polarity. It influences the invasion and metastasis of tumor cells and plays a role in synapse formation in neurons.
| Cat.No | ACP02356 | Target Name | ARF1 |
|---|---|---|---|
| Target Synonyms | ADP Ribosylation Factor 1; ADP-ribosylation factor 1; ARF 1; ARF1; ARF1_HUMAN | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-181aa |
| Mol Weight | 55.7kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P84077 |
|---|
Uniprot Id
P84077
Target Species
Human
Target Name
ARF1
Target Full Name
ADP-ribosylation factor 1
Target Function
GTP-binding protein involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).; (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Target Subcellular Location
Golgi apparatus. Cytoplasm, perinuclear region. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic density. Membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor.
Target Protein Families
Small GTPase superfamily, Arf family
Target Research Area
Signal Transduction
Target Synonyms
ADP Ribosylation Factor 1; ADP-ribosylation factor 1; ARF 1; ARF1; ARF1_HUMAN
Target Background
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
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