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The recombinant Human CALB1 protein is produced by manipulating the expression of specific genes in Yeast cells. A DNA sequence encoding the Human CALB1 protein (2-261aa) is fused to an expression vector and subsequently transformed into Yeast cells. The cells that successfully integrate the vector are selected and cultured to induce the expression of the desired protein. A C-terminal 6xHis tag is attached to the protein. The recombinant Human CALB1 protein protein is then purified using affinity purification, achieving a purity level of over 85%, as confirmed by SDS-PAGE analysis.
The recombinant Human CALB1 protein is produced by manipulating the expression of specific genes in Yeast cells. A DNA sequence encoding the Human CALB1 protein (2-261aa) is fused to an expression vector and subsequently transformed into Yeast cells. The cells that successfully integrate the vector are selected and cultured to induce the expression of the desired protein. A C-terminal 6xHis tag is attached to the protein. The recombinant Human CALB1 protein protein is then purified using affinity purification, achieving a purity level of over 85%, as confirmed by SDS-PAGE analysis.
| Cat.No | ACP01128 | Target Name | CALB1 |
|---|---|---|---|
| Target Synonyms | (Calbindin D28)(D-28K)(Vitamin D-dependent calcium-binding protein, avian-type) | Form | Liquid or Lyophilized powder |
| Expression System | Yeast | Expression Range | 2-261aa |
| Mol Weight | 30.8 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P05937 |
|---|
Uniprot Id
P05937
Target Species
Human
Target Name
CALB1
Target Full Name
Calbindin
Target Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Target Protein Families
Calbindin family
Target Research Area
Others
Target Synonyms
avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMAN; Calbindin 1 28kDa; Calbindin; Calbindin D28; D 28K; D-28K; D28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL H protein; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D-dependent calcium-binding protein
Target Background
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.
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