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To prepare this Recombinant Human CSPG4 protein, the recombinant DNA was required, which was generated by fusing the CSPG4 gene with N-terminal 10xHis tag and C-terminal Myc tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, Mammalian cell, was needed for this CSPG4 protein production. After purification, a premium CSPG4 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+. CSPG4 (also known as MCSP) is an encoding gene that provides an instruction in making protein named chondroitin sulfate proteoglycan 4 (short name is CSPG4). CSPG4 protein, a member of the chondroitin sulfate proteoglycan family, consists of core protein and chondroitin sulfate side chains. It presents on normal human tissues and plays an important role in cell growth, adhesion, and migration. CSPG4 was first found to be highly expressed in melanoma, so it is also known as melanoma proteoglycan (MPG). It is a phylogenetically conserved tumour antigen that plays an important biological role in human melanoma. Currently, it is used as a marker to diagnose forms with unusual characteristics, such as desmoplastic melanoma.
To prepare this Recombinant Human CSPG4 protein, the recombinant DNA was required, which was generated by fusing the CSPG4 gene with N-terminal 10xHis tag and C-terminal Myc tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, Mammalian cell, was needed for this CSPG4 protein production. After purification, a premium CSPG4 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+. CSPG4 (also known as MCSP) is an encoding gene that provides an instruction in making protein named chondroitin sulfate proteoglycan 4 (short name is CSPG4). CSPG4 protein, a member of the chondroitin sulfate proteoglycan family, consists of core protein and chondroitin sulfate side chains. It presents on normal human tissues and plays an important role in cell growth, adhesion, and migration. CSPG4 was first found to be highly expressed in melanoma, so it is also known as melanoma proteoglycan (MPG). It is a phylogenetically conserved tumour antigen that plays an important biological role in human melanoma. Currently, it is used as a marker to diagnose forms with unusual characteristics, such as desmoplastic melanoma.
| Cat.No | ACP05197 | Target Name | CSPG4 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Mammalian cell |
| Expression Range | 1583-2224aa | Mol Weight | 73.2 kDa |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q6UVK1 |
|---|
Uniprot Id
Q6UVK1
Target Species
Human
Target Name
CSPG4
Target Full Name
Chondroitin sulfate proteoglycan 4
Target Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Target Subcellular Location
Cell membrane; Single-pass type I membrane protein; Extracellular side. Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side. Cell surface.
Target Tissue Specificity
Detected only in malignant melanoma cells.
Target Research Area
Cancer
Target Synonyms
4732461B14Rik; AN2; AN2 proteoglycan; Chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; Cspg4; Cspg4 chondroitin sulfate proteoglycan 4; CSPG4_HUMAN; HMW-MAA; HSN tumor-specific antigen; Kiaa4232; MCSP; MCSPG; MEL-CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; MELCSPG; MSK16; NG2
Target Background
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
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