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In expressing the recombinant Human ADAM33 protein, baculovirus cells are transfected with a DNA expression vector that harbors the gene for the desired protein (30-701aa). The cultured cells then express the intended protein. The protein is equipped with a C-terminal 6xHis tag. The recombinant Human ADAM33 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 90%, as confirmed by SDS-PAGE.
In expressing the recombinant Human ADAM33 protein, baculovirus cells are transfected with a DNA expression vector that harbors the gene for the desired protein (30-701aa). The cultured cells then express the intended protein. The protein is equipped with a C-terminal 6xHis tag. The recombinant Human ADAM33 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 90%, as confirmed by SDS-PAGE.
| Cat.No | ACP01626 | Target Name | ADAM33 |
|---|---|---|---|
| Target Synonyms | (ADAM 33) | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 30-701aa |
| Mol Weight | 78.0 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9BZ11 |
|---|
Uniprot Id
Q9BZ11
Target Species
Human
Target Name
ADAM33
Target Full Name
Disintegrin and metalloproteinase domain-containing protein 33
Target Involvement
Asthma (ASTHMA)
Target Subcellular Location
Membrane; Single-pass type I membrane protein.
Target Tissue Specificity
Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
Target Research Area
Cell Biology
Target Synonyms
A disintegrin and metalloprotease 33; A disintegrin and metalloproteinase domain 33; ADA33_HUMAN; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; Disintegrin and metalloproteinase domain-containing protein 33; Disintegrin and reprolysin metalloproteinase family protein; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; Metalloprotease disintegrin; MGC149823; MGC71889; PRO1891; UNQ873
Target Background
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing results in multiple transcript variants encoding different isoforms.
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