Recombinant Human DNA polymerase iota (POLI)

Uniprot Id

Q9UNA4

Target Species

Human

Target Name

POLI

Target Full Name

DNA polymerase iota

Target Function

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Target Subcellular Location

Nucleus.

Target Protein Families

DNA polymerase type-Y family

Target Tissue Specificity

Ubiquitous. Highly expressed in testis.

Target Synonyms

DNA polymerase iota; Eta 2; Eta2; POLI; POLI_HUMAN; Polymerase (DNA directed) iota; RAD 30B; RAD30 homolog B; RAD30, S. cerevisiae, homolog of; RAD30B; RAD3OB

Target Background

The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.