Recombinant Human E3 ubiquitin-protein ligase RNF146 (RNF146)

Cat.No	ACP10263	Target Name	RNF146
Target Synonyms	Dactylidin; dJ351K20.1 ; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1	Form	Lyophilized powder
Expression System	Custom Production. Please inquire and provide the desire expression system.	Expression Range	1-359
Protein Length	Full length protein	Purity	>85% (SDS-PAGE)
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP10263

Target Name

RNF146

Target Synonyms

Dactylidin; dJ351K20.1 ; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1

Form

Lyophilized powder

Expression System

Custom Production. Please inquire and provide the desire expression system.

Expression Range

1-359

Protein Length

Full length protein

Purity

>85% (SDS-PAGE)

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q9NTX7

Background Information

Uniprot Id

Q9NTX7
Target Species

Human
Target Name

RNF146
Target Full Name

E3 ubiquitin-protein ligase RNF146
Target Function

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair
Target Involvement

Defects in RNF146 are a cause of susceptibility to breast cancer.
Target Subcellular Location

Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding.
Target Tissue Specificity

Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
Target Synonyms

Dactylidin; dJ351K20.1 ; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1

Recombinant Human E3 ubiquitin-protein ligase RNF146 (RNF146)

Specifications

Immunogen Information

Inquire Recombinant Human E3 ubiquitin-protein ligase RNF146 (RNF146) Now

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