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Inserting the gene encoding the Human LANCL1 protein (2-399aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human LANCL1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human LANCL1 protein, revealing a purity level exceeding 85%.
Inserting the gene encoding the Human LANCL1 protein (2-399aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human LANCL1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human LANCL1 protein, revealing a purity level exceeding 85%.
| Cat.No | ACP05174 | Target Name | LANCL1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-399aa | Mol Weight | 52.6 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O43813 |
|---|
Uniprot Id
O43813
Target Species
Human
Target Name
LANCL1
Target Full Name
Glutathione S-transferase LANCL1
Target Function
Functions as glutathione transferase. Catalyzes conjugation of the glutathione (GSH) to artificial substrates 1-chloro-2,4-dinitrobenzene (CDNB) and p-nitrophenyl acetate. Mitigates neuronal oxidative stress during normal postnatal development and in response to oxidative stresses probably through GSH antioxidant defense mechanism. May play a role in EPS8 signaling. Binds glutathione.
Target Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Target Protein Families
LanC-like protein family
Target Tissue Specificity
Detected in erythrocytes, brain, kidney, testis, ovary, heart, lung, placenta and spleen (at protein level). Ubiquitous. Strongly expressed in brain, spinal cord, pituitary gland, kidney, heart, skeletal muscle, pancreas, ovary and testis.
Target Research Area
Cardiovascular
Target Synonyms
40 kDa erythrocyte membrane protein; G protein-coupled receptor 69A; GPR69A; LanC (bacterial lantibiotic synthetase component C) like 1; LanC lantibiotic synthetase component C like 1 (bacterial); LanC-like protein 1; LANC1_HUMAN; LANCL1; p40
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