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The gene responsible for the Human GOLM1 protein (36-401aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human GOLM1 protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Human GOLM1 protein, revealing a purity greater than 90%.
The gene responsible for the Human GOLM1 protein (36-401aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human GOLM1 protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Human GOLM1 protein, revealing a purity greater than 90%.
| Cat.No | ACP02881 | Target Name | GOLM1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 36-401aa | Mol Weight | 45.6kDa |
| Protein Length | Partial | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8NBJ4 |
|---|
Uniprot Id
Q8NBJ4
Target Species
Human
Target Name
GOLM1
Target Full Name
Golgi membrane protein 1
Target Function
Unknown. Cellular response protein to viral infection.
Target Subcellular Location
Golgi apparatus, cis-Golgi network membrane; Single-pass type II membrane protein. Note=Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
Target Protein Families
GOLM1/CASC4 family
Target Tissue Specificity
Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in n
Target Research Area
Signal Transduction
Target Synonyms
bA379P1.3; C9orf155; Chromosome 9 open reading frame 155; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2; Golgi protein 73 kD ; Golgi protein 73kD ; GOLM 1; GOLM1; GOLM1_HUMAN; GOLPH 2; GOLPH2; GP 73; GP73; PSEC0257
Target Background
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.
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