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To achieve the expression of the recombinant Human TMEM219 protein in e.coli cells, a DNA fragment encoding the Human TMEM219 protein (39-204aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the TMEM219 protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human TMEM219 protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Human TMEM219 protein is greater than 90%, measured by SDS-PAGE.
To achieve the expression of the recombinant Human TMEM219 protein in e.coli cells, a DNA fragment encoding the Human TMEM219 protein (39-204aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the TMEM219 protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human TMEM219 protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Human TMEM219 protein is greater than 90%, measured by SDS-PAGE.
| Cat.No | ACP01985 | Target Name | TMEM219 |
|---|---|---|---|
| Target Synonyms | Transmembrane protein 219 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 39-204aa |
| Mol Weight | 22.7 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q86XT9 |
|---|
Uniprot Id
Q86XT9
Target Species
Human
Target Name
TMEM219
Target Full Name
Insulin-like growth factor-binding protein 3 receptor
Target Function
Cell death receptor specific for IGFBP3, may mediate caspase-8-dependent apoptosis upon ligand binding.
Target Subcellular Location
Cell membrane; Single-pass membrane protein.
Target Tissue Specificity
Widely expressed in normal tissues but suppressed in prostate and breast tumor.
Target Research Area
Cardiovascular
Target Synonyms
TMEM219; Insulin-like growth factor-binding protein 3 receptor; IGFBP-3R; Transmembrane protein 219
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