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The recombinant Human ITIH3 protein expression involves the transfection of e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (512-651aa). Following transfection, the cells are cultured to induce the expression of the desired protein. The protein is equipped with a N-terminal 10xHis-GST tag and C-terminal Myc tag. The recombinant Human ITIH3 protein is collected and purified from the cell lysate through affinity purification, achieving a purity greater than 85%, as determined by SDS-PAGE.
The recombinant Human ITIH3 protein expression involves the transfection of e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (512-651aa). Following transfection, the cells are cultured to induce the expression of the desired protein. The protein is equipped with a N-terminal 10xHis-GST tag and C-terminal Myc tag. The recombinant Human ITIH3 protein is collected and purified from the cell lysate through affinity purification, achieving a purity greater than 85%, as determined by SDS-PAGE.
| Cat.No | ACP01536 | Target Name | ITIH3 |
|---|---|---|---|
| Target Synonyms | (ITI heavy chain H3)(ITI-HC3)(Inter-alpha-inhibitor heavy chain 3)(Serum-derived hyaluronan-associated protein)(SHAP) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 512-651aa |
| Mol Weight | 51.2 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q06033 |
|---|
Uniprot Id
Q06033
Target Species
Human
Target Name
ITIH3
Target Full Name
Inter-alpha-trypsin inhibitor heavy chain H3
Target Function
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Target Subcellular Location
Secreted.
Target Protein Families
ITIH family
Target Research Area
Others
Target Synonyms
(ITI heavy chain H3)(ITI-HC3)(Inter-alpha-inhibitor heavy chain 3)(Serum-derived hyaluronan-associated protein)(SHAP)
Target Background
This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3.
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