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The synthesis of the recombinant plasmid containing the gene encoding the Human LGR5 protein (22-561aa) is the first step to produce the recombinant Human LGR5 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal myc tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human LGR5 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human LGR5 protein, indicating a purity level exceeding 90%.
The synthesis of the recombinant plasmid containing the gene encoding the Human LGR5 protein (22-561aa) is the first step to produce the recombinant Human LGR5 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal myc tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human LGR5 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human LGR5 protein, indicating a purity level exceeding 90%.
| Cat.No | ACP02039 | Target Name | LGR5 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 22-561aa | Mol Weight | 65.4kDa |
| Protein Length | Extracellular Domain | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O75473 |
|---|
Uniprot Id
O75473
Target Species
Human
Target Name
LGR5
Target Full Name
Leucine-rich repeat-containing G-protein coupled receptor 5
Target Function
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Target Subcellular Location
Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein.
Target Protein Families
G-protein coupled receptor 1 family
Target Tissue Specificity
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of th
Target Research Area
Others
Target Synonyms
FEX; G protein coupled receptor 49; G protein coupled receptor 67; g protein-coupled receptor fex; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GPCR GPR49; GPR 49; GPR 67; GPR49; GPR67; GRP 49; GRP49; HG 38; HG38; Leucine rich repeat containing G protein coupled receptor 5 ; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; LGR5; LGR5_HUMAN; MGC117008; Orphan G protein coupled receptor HG38
Target Background
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
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