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The process for producing recombinant Human MUC5B protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human MUC5B protein (4186-4295aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human MUC5B protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
The process for producing recombinant Human MUC5B protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human MUC5B protein (4186-4295aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human MUC5B protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
| Cat.No | ACP01794 | Target Name | MUC5B |
|---|---|---|---|
| Target Synonyms | MUC-5B;Cervical mucin;High molecular weight salivary mucin MG1;Mucin-5 subtype B, tracheobronchial;Sublingual gland mucin | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 4186-4295aa |
| Mol Weight | 18.9 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9HC84 |
|---|
Uniprot Id
Q9HC84
Target Species
Human
Target Name
MUC5B
Target Full Name
Mucin-5B
Target Function
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Target Involvement
Pulmonary fibrosis, idiopathic (IPF)
Target Subcellular Location
Secreted.
Target Tissue Specificity
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
Target Research Area
Cell Biology
Target Synonyms
2300002I04Rik; A130042M24; AV085033; Cervical mucin; Cervical mucin MUC5B; High molecular weight salivary mucin MG1; MG1; MUC-5B; MUC5; MUC5B; MUC5B_HUMAN; MUC9; Mucin 5 subtype B, tracheobronchial; Mucin 5B; Mucin 5B oligomeric mucus/gel forming; Mucin-5 subtype B; Mucin-5B; Sublingual gland mucin; tracheobronchial
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