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This Human NME4 recombinant protein was produced in E.coli, where the gene sequence encoding Human NME4 (1-187aa) was expressed with the N-terminal GST tag. The purity of this NME4 protein was greater than 90% by SDS-PAGE.NME4 is an enzyme located within the mitochondria, and it is associated with nucleotide metabolism and cellular energy production. Specifically, NME4 catalyzes the reaction of nucleotide diphosphorylation, converting a nucleotide monophosphate into a nucleotide diphosphate. This is an important biochemical process that occurs within mitochondria and is closely related to the generation of adenosine triphosphate (ATP). The primary biological role of NME4 is to maintain the production of ATP within the mitochondria. ATP is the primary energy molecule inside cells, and it plays a crucial role in various biological processes, including muscle contraction, cell signal transduction, and cell division. Therefore, NME4 is essential for maintaining normal cellular functions and survival by ensuring an adequate supply of ATP.
This Human NME4 recombinant protein was produced in E.coli, where the gene sequence encoding Human NME4 (1-187aa) was expressed with the N-terminal GST tag. The purity of this NME4 protein was greater than 90% by SDS-PAGE.NME4 is an enzyme located within the mitochondria, and it is associated with nucleotide metabolism and cellular energy production. Specifically, NME4 catalyzes the reaction of nucleotide diphosphorylation, converting a nucleotide monophosphate into a nucleotide diphosphate. This is an important biochemical process that occurs within mitochondria and is closely related to the generation of adenosine triphosphate (ATP). The primary biological role of NME4 is to maintain the production of ATP within the mitochondria. ATP is the primary energy molecule inside cells, and it plays a crucial role in various biological processes, including muscle contraction, cell signal transduction, and cell division. Therefore, NME4 is essential for maintaining normal cellular functions and survival by ensuring an adequate supply of ATP.
| Cat.No | ACP03483 | Target Name | NME4 |
|---|---|---|---|
| Target Synonyms | homolog 4; Nucleoside diphosphate kinase D; Nucleoside diphosphate kinase, metastatic inhibition factor NM23H4; mitochondrial; NDK; NDKM_HUMAN; NDP kinase; NDP kinase D; NDP kinase, mitochondrial, mitochondrial; NDPK D; NDPKD; nm23 H4; nm23-H4; NM23D; NM23H4; Nm23M4; NME/NM23 nucleoside diphosphate kinase 4; NME4; Non metastatic cells 4 protein expressed in; Non metastatic protein 23, mitochondrial; Nucleoside diphosphate kinase | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-187aa |
| Mol Weight | 44.3kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O00746 |
|---|
Uniprot Id
O00746
Target Species
Human
Target Name
NME4
Target Full Name
Nucleoside diphosphate kinase, mitochondrial
Target Function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis. Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity. Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration. Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria. In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling.
Target Subcellular Location
Mitochondrion intermembrane space; Peripheral membrane protein. Mitochondrion matrix.
Target Protein Families
NDK family
Target Tissue Specificity
Widely distributed. Found at very high levels in prostate, heart, liver, small intestine, and skeletal muscle tissues, and in low amounts in the brain and in blood leukocytes.
Target Research Area
Signal Transduction
Target Synonyms
metastatic inhibition factor NM23H4; mitochondrial; NDK; NDKM_HUMAN; NDP kinase; NDP kinase D; NDP kinase, mitochondrial; NDPK D; NDPKD; nm23 H4; nm23-H4; NM23D; NM23H4; Nm23M4; NME/NM23 nucleoside diphosphate kinase 4; NME4; Non metastatic cells 4 protein expressed in; Non metastatic protein 23, homolog 4; Nucleoside diphosphate kinase D; Nucleoside diphosphate kinase, mitochondrial; Nucleoside diphosphate kinase, mitochondrial
Target Background
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).
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