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The plasmid vector incorporates the gene encoding the Human LOX protein (169-417aa), generating recombinant plasmid, which is then introduced into baculovirus cells. Selection of positive baculovirus cells is based on their capacity to withstand a particular antibiotic. Subsequently, the baculovirus cells containing the recombinant plasmid are cultivated under conditions that facilitate the expression of the Human LOX gene. The protein carries a N-terminal MBP tag and C-terminal 6xHis tag. Following expression, affinity purification is employed to isolate and purify the recombinant Human LOX protein from the cell lysate. Denaturing SDS-PAGE is used to resolve the resulting recombinant protein, enabling an estimation of its purity, exceeding 85%.
The plasmid vector incorporates the gene encoding the Human LOX protein (169-417aa), generating recombinant plasmid, which is then introduced into baculovirus cells. Selection of positive baculovirus cells is based on their capacity to withstand a particular antibiotic. Subsequently, the baculovirus cells containing the recombinant plasmid are cultivated under conditions that facilitate the expression of the Human LOX gene. The protein carries a N-terminal MBP tag and C-terminal 6xHis tag. Following expression, affinity purification is employed to isolate and purify the recombinant Human LOX protein from the cell lysate. Denaturing SDS-PAGE is used to resolve the resulting recombinant protein, enabling an estimation of its purity, exceeding 85%.
| Cat.No | ACP02330 | Target Name | LOX |
|---|---|---|---|
| Target Synonyms | lox; LYOX; LYOX_HUMAN; Lysyl oxidase; MGC105112; Protein lysine 6 oxidase; Protein-lysine 6-oxidase | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 169-417aa |
| Mol Weight | 73.0 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P28300 |
|---|
Uniprot Id
P28300
Target Species
Human
Target Name
LOX
Target Full Name
Protein-lysine 6-oxidase
Target Function
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
Target Involvement
Aortic aneurysm, familial thoracic 10 (AAT10)
Target Subcellular Location
Secreted. Secreted, extracellular space.
Target Protein Families
Lysyl oxidase family
Target Tissue Specificity
Heart, placenta, skeletal muscle, kidney, lung and pancreas.
Target Research Area
Cardiovascular, Signal Transduction
Target Synonyms
lox; LYOX; LYOX_HUMAN; Lysyl oxidase; MGC105112; Protein lysine 6 oxidase; Protein-lysine 6-oxidase
Target Background
This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. In addition, defects in this gene have been linked with predisposition to thoracic aortic aneurysms and dissections.
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