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The formation of the recombinant plasmid by incorporating the gene encoding the Human RAB5A protein (1-215aa) into a plasmid vector initiates the generation of the recombinant Human RAB5A protein. Transforming the recombinant plasmid into e.coli cells and then selecting the positive e.coli cells based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that stimulate the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant Human RAB5A protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human RAB5A protein, revealing a purity level exceeding 90%.
The formation of the recombinant plasmid by incorporating the gene encoding the Human RAB5A protein (1-215aa) into a plasmid vector initiates the generation of the recombinant Human RAB5A protein. Transforming the recombinant plasmid into e.coli cells and then selecting the positive e.coli cells based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that stimulate the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant Human RAB5A protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human RAB5A protein, revealing a purity level exceeding 90%.
| Cat.No | ACP04043 | Target Name | RAB5A |
|---|---|---|---|
| Target Synonyms | RAB 5; RAB 5A; RAB5A; RAB5A member RAS oncogene family; RAB5A_HUMAN; RAS associated protein RAB5A; Ras related protein Rab 5A; Ras-related protein Rab-5A | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-215aa |
| Mol Weight | 39.7kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P20339 |
|---|
Uniprot Id
P20339
Target Species
Human
Target Name
RAB5A
Target Full Name
Ras-related protein Rab-5A
Target Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
Target Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Endosome membrane.
Target Protein Families
Small GTPase superfamily, Rab family
Target Research Area
Developmental Biology
Target Synonyms
RAB 5; RAB 5A; RAB5A; RAB5A member RAS oncogene family; RAB5A_HUMAN; RAS associated protein RAB5A; Ras related protein Rab 5A; Ras-related protein Rab-5A
Target Background
Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis; early endosome to late endosome transport; and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane; nucleoplasm; and terminal bouton.
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