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In expressing the recombinant Human SPINK5 protein, e.coli cells are transfected with a DNA expression vector that harbors the gene for the desired protein (722-847aa). The cultured cells then express the intended protein. The protein is equipped with a N-terminal 10xHis-GST tag and C-terminal Myc tag. The recombinant Human SPINK5 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 85%, as confirmed by SDS-PAGE.
In expressing the recombinant Human SPINK5 protein, e.coli cells are transfected with a DNA expression vector that harbors the gene for the desired protein (722-847aa). The cultured cells then express the intended protein. The protein is equipped with a N-terminal 10xHis-GST tag and C-terminal Myc tag. The recombinant Human SPINK5 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 85%, as confirmed by SDS-PAGE.
| Cat.No | ACP01656 | Target Name | SPINK5 |
|---|---|---|---|
| Target Synonyms | (Lympho-epithelial Kazal-type-related inhibitor)(LEKTI) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 722-847aa |
| Mol Weight | 49.5 kDa | Protein Length | Partial of Isoform l |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9NQ38 |
|---|
Uniprot Id
Q9NQ38
Target Species
Human
Target Name
SPINK5
Target Full Name
Serine protease inhibitor Kazal-type 5
Target Function
Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.
Target Involvement
Netherton syndrome (NETH)
Target Subcellular Location
Secreted.
Target Tissue Specificity
Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.
Target Research Area
Cell Biology
Target Synonyms
Hemofiltrate peptide HF7665; ISK5_HUMAN; LEKTI; Lympho-epithelial Kazal-type-related inhibitor; Lymphoepithelial Kazal type related inhibitor; NETS; NS; Serine peptidase inhibitor Kazal type 5; SPINK5; VAKTI
Target Background
This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.
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