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The recombinant Human CYP17A1 was expressed with the amino acid range of 1-508. The calculated molecular weight for this CYP17A1 protein is 84.4 kDa. This protein is generated in a e.coli-based system. The CYP17A1 gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant CYP17A1 protein during the following stages.Steroid 17-alpha-hydroxylase/17,20 lyase, encoded by the CYP17A1 gene, is a critical enzyme involved in steroidogenesis. This multifunctional cytochrome P450 enzyme plays a key role in both the adrenal cortex and gonads, where it participates in the biosynthesis of steroid hormones. In the adrenal glands, CYP17A1 catalyzes two essential reactions in the steroidogenic pathway: the 17-alpha-hydroxylation of pregnenolone and progesterone, and the subsequent 17,20 lyase cleavage, leading to the production of androgens, such as dehydroepiandrosterone (DHEA) and androstenedione. These androgens serve as precursors for the synthesis of sex hormones like testosterone and estrogen. CYP17A1's activities are crucial for the development and maintenance of secondary sexual characteristics and overall reproductive function. Dysfunction of CYP17A1 can lead to disorders of sex development and hormonal imbalances. Understanding the regulation of CYP17A1 is vital in the context of endocrine disorders and therapeutic interventions targeting steroid hormone production.
The recombinant Human CYP17A1 was expressed with the amino acid range of 1-508. The calculated molecular weight for this CYP17A1 protein is 84.4 kDa. This protein is generated in a e.coli-based system. The CYP17A1 gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant CYP17A1 protein during the following stages.Steroid 17-alpha-hydroxylase/17,20 lyase, encoded by the CYP17A1 gene, is a critical enzyme involved in steroidogenesis. This multifunctional cytochrome P450 enzyme plays a key role in both the adrenal cortex and gonads, where it participates in the biosynthesis of steroid hormones. In the adrenal glands, CYP17A1 catalyzes two essential reactions in the steroidogenic pathway: the 17-alpha-hydroxylation of pregnenolone and progesterone, and the subsequent 17,20 lyase cleavage, leading to the production of androgens, such as dehydroepiandrosterone (DHEA) and androstenedione. These androgens serve as precursors for the synthesis of sex hormones like testosterone and estrogen. CYP17A1’s activities are crucial for the development and maintenance of secondary sexual characteristics and overall reproductive function. Dysfunction of CYP17A1 can lead to disorders of sex development and hormonal imbalances. Understanding the regulation of CYP17A1 is vital in the context of endocrine disorders and therapeutic interventions targeting steroid hormone production.
| Cat.No | ACP04248 | Target Name | CYP17A1 |
|---|---|---|---|
| Target Synonyms | 20 lyase; Steroid 17 alpha monooxygenase; Steroid 17-alpha-hydroxylase/17; Steroid 17-alpha-monooxygenase | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-508aa |
| Mol Weight | 84.4kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P05093 |
|---|
Uniprot Id
P05093
Target Species
Human
Target Name
CYP17A1
Target Full Name
Steroid 17-alpha-hydroxylase/17,20 lyase
Target Function
A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Target Involvement
Adrenal hyperplasia 5 (AH5)
Target Subcellular Location
Endoplasmic reticulum membrane. Microsome membrane.
Target Protein Families
Cytochrome P450 family
Target Research Area
Metabolism, Cardiovascular
Target Synonyms
20 lyase; CP17A_HUMAN; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 family 17; Cytochrome P450 family 17 subfamily A polypeptide 1; Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia; Cytochrome p450 XVIIA1; Cytochrome P450-C17; Cytochrome P450c17; OTTHUMP00000020382; P450 C17; P450c17; S17AH; Steroid 17 alpha hydroxylase/17,20 lyase; Steroid 17 alpha monooxygenase; Steroid 17-alpha-hydroxylase/17; Steroid 17-alpha-monooxygenase
Target Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17, 20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17, 20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
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